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SOLVED: I need to calculate the primer concentration after PCR cycle. My DNA segment (798 bp) is amplified by PCR. The 50 uL mixture of PCR reaction. Primer concentration is 2 uM.
SOLVED: Scientific papers provide the final concentrations of ingredients in PCRs (Polymerase Chain Reactions). Aksöyek et al. 2016 set up 50 ul reactions for the PCRs using the primer pair HCO2198–LCO1490 (Folmer
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Barrick Lab :: PrimerEfficiencyqPCR
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Solved] Calculate a reaction mixture for PCR to amplify the K1 CDS, given... | Course Hero
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